Tissue on the African-American brains who are ApoE4 carriers

 
   Lead Investigator:    Jeffrey Vance
   Institution      :    University of Miami
   E-Mail           :    JVance@med.miami.edu
   Proposal ID      :    968



   Proposal Description:
   We will establish iPSC from individuals homozygous for the African #9494 admixture block, the NHW #9494 admixture-block and APOE #9493 and use directed differentiation with cell type specific transcription factors for neurons, astrocytes and microglia. We will evaluate cellular differences using well-established assays to Abeta in these cell types, as well in hippocampus and temporal lobes of AA brains with each #9494 admixture block.  b) We will perform RNA-seq analyses including differential expression, splicing changes and pathway analyses along with cellular assays to detect key differences between the two blocks and help direct CRISPR studies in Aim 4.